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• 荧光定量PCR引物为什么要跨内含子设计,大家都说是为了避免提RNA时残留基因组的干扰,但是即使跨了内含子,假如有基因组残留,Real time PCR中还是能P出比cDNA大的一条带（含内含子的条带）,探针同样会结合这条大的片段,不会只结合小的片段,还是会干扰定量,除非引物只能扩增cDNA,不能扩增基因组,那就不存在基因组的干扰了.很困惑,请各位指教.
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