英语翻译

英语翻译
Bacillus subtilis natto strain was isolated from commercial natto available in the Australian market.Bacterial cells were cultivated in
a liquid culture constituting 0.5% (w/v) peptone,0.5% (w/v)
glucose and0.05%(w/v) yeast extract for five days before streaking
on tryptic soy agar plates for aseptic spore genesis.Plates were
scrapped after five days and harvested cells were suspended in a
0.9% (w/v) NaCl solution.The spore suspension was kept in a
heated water bath (808C,30 min) to kill the residual vegetative
cells,centrifugedat3000 rpm for 10 min to remove the cell debris,
diluted using 0.9% (w/v) NaCl solution to obtain the standard
spore solution (5.2 \3 0.5 \4 1010 spores/mL).