如何配制1mM Tris PH 7.4 ,0.25mM EDTA啊?
问题描述:
如何配制1mM Tris PH 7.4 ,0.25mM EDTA啊?
答
Tris(碱)12.1mg,加蒸馏水800ml溶解,加EDTA.2Na 84.562mg(EDTA.4Na96.07mg)溶解,用4MHCl调pH7.4,定容1L
建议配置10倍的浓缩液,使用时10倍稀释即可