设计PCR引物时Tm温度有没有具体要求?比如说,Tm必须小于72度,或大于72度?
问题描述：
设计PCR引物时Tm温度有没有具体要求?比如说,Tm必须小于72度,或大于72度?
答
The best temperature for Taq polymerase is 72C. If your primer Tm is higher than 72C, then the polymerase would not work well, since the primer will not anneal to the template. If your primer Tm is lo...