英语翻译The sample digest was then purified until a minimal proteininterface was obtained by a series of equal volumeextractions of 0.1 M Tris pH8 equilibrated phenol,phenol/chloroform/isoamylalcohol (24:24:1) and chloroform/isoamylalcohol (24:1).0.2 volumes of 10M ammonium acetate and 2 volumes of absolute ethanolwere added to precipitate the DNA from the final aqueous phase.求专业 非专业人士请绕行！
英语翻译
The sample digest was then purified until a minimal protein
interface was obtained by a series of equal volume
extractions of 0.1 M Tris pH8 equilibrated phenol,phenol/chloroform/isoamylalcohol (24:24:1) and chloroform/isoamylalcohol (24:1).0.2 volumes of 10M ammonium acetate and 2 volumes of absolute ethanol
were added to precipitate the DNA from the final aqueous phase.
求专业 非专业人士请绕行！
当时的样品净化直到消化低蛋白
接口得到一系列平等的体积
三pH8提取物为0.1米equilibrated苯酚、苯酚/氯仿/ isoamylalcohol(24:24:1)、氯仿/ / isoamylalcohol(24:1)。0.2卷10米的醋酸铵和上下卷绝对的乙醇
添加沉淀DNA从最终的水相吗
+
消化好的样品用于下步纯化。
使用等体积的0.1M Tris（pH8）平衡酚、酚/氯仿/异戊醇（24:24:1）和氯仿/异戊醇（24:1）分别抽提后，最终使得水相和有机相之间有少量蛋白的分界面。
吸取水相，然后加入0.2体积的乙酸钠（10M）和2体积的无水乙醇沉淀DNA。
饭粒啊,看到好友动态,才知道你到这里来求翻译,以后直接找我就好了（翻译的时候我给你加了一些词,有助于理解）（蛋白）消化后的样本用用以下步骤提纯,以尽量减少残留蛋白的干扰：依次用等体积一下溶液萃取:酚（事先用...
样品消化后，直到一个最小的蛋白质纯化界面得到一系列的平等提取的0.1米三ph8平衡酚，酚/氯仿/isoamylalcohol（24:24:1）和氯仿/isoamylalcohol（1）。0.2卷2卷1000醋酸铵和无水乙醇增加了沉淀脱氧核糖核酸从最后水相。