PCR体系怎么配PCR was performed in a reaction volume of 25 ll containing PCR reaction buffer (50 mM KCl,10 mM Tris-HCl,2.5 mMMgCl2,pH 8.3),200μMdNTPs,0.2 μM invA primers,0.2μM sefA primers,and 0.5 μM pefA primers,2.5U of Taq DNA polymerase (MBI Fermentas),and 0.2μl of DNA template.这段话怎么翻译啊,这个PCR体系中的10*buffer、dNTP、引物invA、sefA及pefA的体积各是多少,怎么算的啊
问题描述：
PCR体系怎么配
PCR was performed in a reaction volume of 25 ll containing PCR reaction buffer (50 mM KCl,10 mM Tris-HCl,2.5 mMMgCl2,pH 8.3),200μMdNTPs,0.2 μM invA primers,0.2μM sefA primers,and 0.5 μM pefA primers,2.5U of Taq DNA polymerase (MBI Fermentas),and 0.2μl of DNA template.
这段话怎么翻译啊,这个PCR体系中的10*buffer、dNTP、引物invA、sefA及pefA的体积各是多少,怎么算的啊
答
在25ul的反应体系中配置,PCR Buffer(组成：50 mM KCl,10 mM Tris-HCl,2.5 mMMgCl2,pH 8.3),200μM dNTPs(200μM是指25ul体系中的dNTPs的终浓度,下面一样),0.2 μM invA primers,-----0.2 μM终浓度的配对链引物(即上...