英语翻译以广州华力园艺公司引进的品种黑叶观音莲（Herba MonachosoriHenryi）作为试验材料,研究了外植体与消毒时间对外植体培养成活率及污染率的影响.同时还研究了培养基配方对芽增殖诱导的影响,进一步了解激素浓度和矿物质元素对试验材料长势的影响.结果表明,外植体消毒时间为48min时成活率较高及污染率,死亡率也较低,最适合进行原种制作；减少NAA的浓度、6-BA的浓度、降低矿物质元素的浓度,对增殖系数的提高均有一定的抑制作用,最后得出6-BA和IAA组合对观音莲不定芽增殖有促进作用,其最适的培养基为MS+6-BA 3.00 mg/L+NAA 1.00 mg/L.
英语翻译
以广州华力园艺公司引进的品种黑叶观音莲（Herba MonachosoriHenryi）作为试验材料,研究了外植体与消毒时间对外植体培养成活率及污染率的影响.同时还研究了培养基配方对芽增殖诱导的影响,进一步了解激素浓度和矿物质元素对试验材料长势的影响.结果表明,外植体消毒时间为48min时成活率较高及污染率,死亡率也较低,最适合进行原种制作；减少NAA的浓度、6-BA的浓度、降低矿物质元素的浓度,对增殖系数的提高均有一定的抑制作用,最后得出6-BA和IAA组合对观音莲不定芽增殖有促进作用,其最适的培养基为MS+6-BA 3.00 mg/L+NAA 1.00 mg/L.
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The introduction of Guangzhou Huali horticultural varieties. A Buddism godness Guanyin Lotus (Herba MonachosoriHenryi) as experimental materials, the explants and the disinfection time outside the plant body culture survival rate and the rate of pollution. At the same time also studied the effect of culture medium on the induction of bud proliferation, to further understand the effects of hormone concentrations and mineral elements on the growth of test materials. The results show that, the explant sterilization time is 48min when the survival rate was higher and the contamination rate, lower mortality rate, the most suitable for seed production; reduce the concentration of NAA, 6-BA concentration, reduce the mineral element concentrations, the inhibitory effect on the proliferation coefficient were increased, the 6-BA and IAA combination on a Buddism godness Guanyin lotus don't shoot proliferation, the optimal medium was MS+6-BA 3 mg/L+NAA 1 mg/L.