WPM培养基和MS培养基的区别
WPM培养基和MS培养基的区别
成分区别
木本植物用培养基(WPM,Woody Plant medium)
1.无机盐
NH4N03 400mg/
Ca(NO3)2.4H2O 556mg/L
K2SO4 990 mg/L
CaCl2?2H2O 96 mg/L
KH2PO4 170 mg/L
Na2MoO4?2H2O 0.25 mg/L
MgS()4?7H2O 370 mg/L
MnSO4?H2O 22.4mg/L
ZnSO4?7H2O 8.6mg/L
CuSO4?5H2O 0.25mg/L
FeSO4?7H2O 27.8 mg/L
Na2-EDTA 37.3 mg/L
2.有机物
肌醇 100 mg/L
维生素B1 1.0mg/L
烟酸 0.5 mg/L
维生素B6 0.5 mg/L
甘氨酸 2.0 mg/L
蔗糖 20 g/L
琼脂 6g/L
pH 5.2
MS培养基RM—62(Murashige & Skoog (1962))
1无机盐
NH4N03 l 650mg/L
KNO3 1900 mg/L
CaCl2·2H2O 440 mg/L
MgSO4·7H2O 370 mg/L
KH2PO4 170 mg/L
Na2一EDTA 37.3 mg/L
FeSO4·7H2O 27.8 mg/L
H3BO3 6.2 mg/L
MnSO4·4H2O 22.3 mg/L
ZnSO4·7H2O 8.6 mg/L
KI 0.83 mg/L
Na2MoO·2H2O 0.25 mg/L
CuSO4·5H2O 0.025 mg/L
CoCl2·6H2O 0.025 mg/L
2有机物质:
肌醇 100 mg/L
烟酸 0.5 mg/L
盐酸吡哆醇(维生素B6) 0.5 mg/L
盐酸硫胺素(维生素B1) 0.1 mg/L
甘氨酸 2.0 mg/L
效果区别:在初代培养过程中,以WPM为基本培养基效果要好,MS培养基效果要差很多.在生根培养过程中,MS培养基和WPM培养基效果基本一样,MS养基对种子萌发及幼苗生长WPM培养基好一些.