pH8.0磷酸钠缓冲溶液咋配啊!
问题描述:
pH8.0磷酸钠缓冲溶液咋配啊!
答
(1)A液(0.2M磷酸二氢钠水溶液):
NaH2PO4•H2O 27.6g,溶于蒸馏水中,稀释至1000ml.
(2)B液(0.2M磷酸氢二钠水溶液):
Na2HPO4•7H2O 53.6g (或Na2HPO4•12 H2O 71.6g或Na2HPO4•2 H2O 35.6g ) 加蒸馏水溶解,加水至1000ml.
(3)不同pH值磷酸盐缓冲液(PB)缓冲液的配制
A液X ml (参照下表) 中,加入B液Y ml,为0.2M PB.若再加蒸馏水至200ml则成0.1M PB.
PH X ml Y ml pH X ml Y ml
5.7 93.5 6.5 6.9 45.0 55.0
5.8 92.0 8.0 7.0 39.0 61.0
5.9 90.0 10.0 7.1 33.0 67.0
6.0 87.7 12.3 7.2 28.0 72.0
6.1 85.0 15.0 7.3 23.0 77.0
6.2 81.5 18.5 7.4 19.0 81.0
6.3 77.5 22.5 7.5 16.0 84.0
6.4 73.5 26.5 7.6 13.0 87.0
6.5 68.5 31.5 7.7 10.0 90.0
6.6 62.5 37.5 7.8 8.5 91.5
6.7 56.5 43.5 7.9 7.0 93.0
6.8 51.0 49.0 8.0 5.3 94.7