生物专业将如下文字翻译文英文,不要谷歌金山在线翻译什么的,急
生物专业将如下文字翻译文英文,不要谷歌金山在线翻译什么的,急
细胞凋亡与肿瘤的发生和发展密切相关,它对维持组织器官的正常形态和功能起着相当重要的作用,在对肿瘤的治疗与预防过程中具有很大的意义.
由于中草药的本质具有很小的毒副作用、且治疗效果稳定等一系列优点,所以,致力于在中草药中筛选抗癌新药已成为新世纪的科研方向.由于白藜芦醇拥有抑菌、消炎、抵抗癌细胞增长等特点,因此在肿瘤治疗方面具有极大的科学研究前景.
目的：本次实验以主要人肺腺癌A549细胞为研究对象,以此来认识白藜芦醇能够诱导肺癌A549细胞凋亡.方法：通过倒置显微镜、荧光显微镜观察不同浓度白藜芦醇作用于A549细胞在24h、48h和72h的细胞生长形态,同时,利用台盼蓝拒染法检测白藜芦醇对A549细胞的生长抑制率,进而确定IC50值,以及细胞的凋亡情况,再通过流式细胞术检测细胞周期和细胞线粒体膜电位的变化.结果证实白藜芦醇作用在人肺癌A549细胞48h时呈现出不同程度的凋亡性状,同时显示量效关系.白藜芦醇诱导A549细胞凋亡作用在48h的最佳浓度分别为110µg/mL、80µg/mL,且IC50值分别为108.002µg/mL、77.462µg/mL.最后经过流式细胞术检测结果可知,白藜芦醇可将A549细胞滞停于S期和G2期,同时线粒体膜电位伴随浓度的增加而逐渐降低.结论：白藜芦醇诱导人肺腺癌A549细胞凋亡,其IC50值分别为108.002μg/mL、77.462μg/mL.线粒体膜电位随浓度增加而降低.并且细胞阻滞于G2期和S期,进而诱导细胞凋亡.
Cell apoptosis and tumor occurrence and development are closely related, it for the maintenance of tissues and organs of normal morphology and function plays an important role in the tumor, treatment and prevention in the process of great significance.
Because the essence herbal has small toxic and side effects, and therapeutic effect is stable and a series of advantages, therefore, is committed to the Chinese herbal medicine screening of anticancer drugs has become the research direction of new century. As a result of resveratrol has antibacterial, anti-inflammatory, the resistance of cancer cells to grow wait for a characteristic, therefore in the treatment of cancer is of great scientific research prospect.
Objective : this experiment in primary human lung adenocarcinoma A549cells as the research object, in order to understand resveratrol induces lung cancer A549 cell apoptosis. Methods: by inverted microscopy, fluorescence microscopy to observe the effects of different concentrations of resveratrol effects on A549cells in 24h,48h and 72h cell growth pattern, at the same time, using the trypan blue dye exclusion assay for the detection of resveratrol on A549 cell growth inhibition rate, and then determine the value of IC50, and cell apoptosis, and then through the flow cytometry in cell cycle and cell mitochondrial membrane potential change. Results show that resveratrol effects in human lung cancer cell A54948h show different degrees of apoptosis traits, while displaying the dose-effect relationship. Resveratrol induced apoptosis of A549in 48h optimal concentrations were 110µ g/mL,80 µ g/mL, and the IC50values were 108.002µ g/mL,77.462 µ g/mL. After the final flow cytometry results, resveratrol may be A549 cell lag stops at S phase and G2 phase, and the mitochondrial membrane potential decreases with concentration. Conclusion: Resveratrol induces apoptosis in human lung adenocarcinoma A549cell apoptosis, the values of IC50were 108.002μ g/mL,77.462 μ g/mL. Mitochondrial membrane potential decreases with increasing concentration. And cell cycle arrest in G2 phase and S phase, and inducing cell apoptosis.我还问了一个类似的问题，你把这个答案粘过去，我把那个分也给你~