动物组织DNA的提取及核酸浓度的测定
问题描述：
动物组织DNA的提取及核酸浓度的测定
为什么要测310nm处的吸光值?细胞裂解液和沉淀液中的EDTA 有什么作用?氯化钠和醋酸钠有什么作用?
答
DNA concentration is measured by OD260,protein usually is OD280.Some proteins have peak absorbency around 310 nm.Therefore it can be used for judging protein contamination.EDTA is able to chelate Mg2+...