英语翻译写完直接放在网上就行了,以下是帮忙翻译内容：摘 要随着生物信息学的发展,表达序列标签（est）在分子标记开发、新基因分离鉴定、基因表达谱分析、基因组功能注释、基因电子克隆等方面具有重要作用.本文简要介绍了表达序列标签(est)技术的原理、技术路线及其在遗传学图谱、物理图谱的构建、分离与鉴定新基因、基因差异表达的研究、比较基因组学的研究、DNA芯片制备等方面的应用概况.综述了表达序列标签(est)的研究现状,并对(est)的应用前景进行了展望.关键字：基因组；基因；研究现状；est

Abstract: With the development of the bioinformatics, EST plays an importent role in nuclear marker-developing, new gene isolation and identification, analysis of gene expression profile, anotation ot genome fuctions, gene silico cloning and so on. This paper introduces the principle of EST technology, technical route and the research in genetic map, physical map, isolating and identifying new gene, gene differential expression, the research in comparative genomics and the general situation of the DNA wafer preparation. This paper sumarizes the research status of EST,and makes an outlook to EST.
Keywords: genome; gene; research status; EST.