TE为什么能溶解DNATE是tris和EDTA的合剂,主要成分应该是EDTA吧,EDTA不是络合剂吗,为什么可以溶解DNA,百思不得其解,
问题描述:
TE为什么能溶解DNA
TE是tris和EDTA的合剂,主要成分应该是EDTA吧,EDTA不是络合剂吗,为什么可以溶解DNA,百思不得其解,
答
DNA is soluable in water.Tris and EDTA provide the protection to DNA.Tris,usually made at pH8.0,is the good pH and condition for stabolizing DNA.EDTA can chelate magnesium.Since most DNase will not function without magnesium,EDTA chelates magnesium,it will disable the DNase possibly presented in the solution.